Estudo comparativo das espécies de <em>Candida</em>: sensibilidade antifúngica e genes de virulência

Authors

  • Camila Aoyama Vieira Universidade Federal do Mato Grosso, campus de Rondonópolis
  • Wérika Weryanne Rosa de Souza Universidade Federal do Mato Grosso, campus de Rondonópolis
  • Janaína Sousa de Lima Universidade Federal do Mato Grosso, campus de Rondonópolis
  • Letícia Silveira Goulart Universidade Federal do Mato Grosso, campus de Rondonópolis

DOI:

https://doi.org/10.20435/multi.v23i54.1709

Keywords:

Candida, sensibilidade antifúngica, Aspartil Proteases Secretórias.

Abstract

O objetivo deste estudo foi comparar a sensibilidade antifúngica e a presença dos genes de virulência SAP1-3 em linhagens de Candida spp. Foram estudados 49 isolados, sendo 30 C. albicans e 19 Candida não-albicans (CNA). A determinação do perfil de sensibilidade foi realizada conforme protocolo do Clinical and Laboratory Standards Institute. Para identificação dos genes SAP1-3, foi utilizado método de PCR. Todas as amostras de C. albicans foram sensíveis ao fluconazol, dentre as CNA 21% foram sensíveis e 68,4% foram SDD, 100% das linhagens de C. albicans e 94,7% de CNA foram sensíveis ao cetoconazol. Para itraconazol, 86,7% dos isolados de C. albicans e 42,1% de CNA foram sensíveis. Os genes mais frequentes nas linhagens de C. albicans foram SAP1 (56,6%,) e SAP2 (40%) seguido de SAP3 (26%). As amostras de CNA apresentaram um predomínio de SAP2 (42,1%), seguido por SAP1 (10,5%), SAP3 não foi identificado. As espécies de Candida apresentam diferenças significativas quanto ao perfil de sensibilidade a azólico e em relação à presença dos genes SAP1-3, sugerindo que esses fatores devem refletir em uma terapêutica e patogênese distintas para estas leveduras.

Author Biographies

Camila Aoyama Vieira, Universidade Federal do Mato Grosso, campus de Rondonópolis

Graduanda, Curso de Enfermagem

Wérika Weryanne Rosa de Souza, Universidade Federal do Mato Grosso, campus de Rondonópolis

Graduanda, Curso de Enfermagem

Janaína Sousa de Lima, Universidade Federal do Mato Grosso, campus de Rondonópolis

Graduanda, Curso de Enfermagem

Letícia Silveira Goulart, Universidade Federal do Mato Grosso, campus de Rondonópolis

Professora, Curso de Enfermagem

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Published

2018-05-08

How to Cite

Vieira, C. A., de Souza, W. W. R., de Lima, J. S., & Goulart, L. S. (2018). Estudo comparativo das espécies de <em>Candida</em>: sensibilidade antifúngica e genes de virulência. Multitemas, 23(54), 169–182. https://doi.org/10.20435/multi.v23i54.1709